Background: S. aureus is a versatile pathogen of humans and animals and causes a wide variety of diseases. Raw milk is an ideal rich media that help and support the growth of the microorganisms and highly susceptible to S. aureus immediately after milking. As camel milk usually consumed in its raw state, the contamination and intoxication of raw milk with respect to pathogenic S. aureus were a common public health problem. Objective: The aim of this study was to determine the prevalence, antibiotic resistance pattern and associated factors of S. aureus in raw camel milk in Babile District, Oromia Region Eastern Ethiopia. Method: A community based cross-sectional study was conducted in Babille district among 350 camels raw milk. Three houndred fifty householders were sellected by using multi-stage sampling technique. Data was collected using a structured questionnaire and 25 mL of raw IX camel milk were collected using sterile plastic test tubes. S.aureus were, identified through culture, gram stain and biochemical tests.Antimicrobial susceptibility testing was, conducted by Kirby-Bauer disc diffusion techniques. A bivariate and multivariable logistic regression was done and P-value ≤0.05 was considered statistically significant. Result: In this study, the overall prevalence of S. aureus in camels raw milk was 14% (95%CI; 0.100- 0.180) which varied between sampling point that ranged from 10.8(udder) uo to 20 %( vendors). Parity [AOR= 3.52, 95%CI: (1.207, 10.277), P= 0.021], drainage condition of milking place [AOR= 4.62, 95%CI: (1.853, 11.557),P=0.001], not hand washing before milking [AOR= 3.94: 95%CI:(1.599,9.716) ,P=0.003] and type of containers used for selling milk [AOR= 8.40, 95%CI: (1.258, 26.068), P= 0.028] were significant predictor of S. aureous prevalence. All 49 S. aureus isolated were subjected against ten antibiotics and showed that high susceptibility to cefoxitin (95.9%), oxacillin (95.9%) and erythromycin (93.9%), while high level of resistance was recorded against tetracycline (81.6%) and penicillin G (81.6%) and the prevalence of multi-drug resistance of S. aureus isolated were 67.34% (n=33/49). Conclusion: The study showed that, the prevalence of multi-drug resistant S. aureus isolated from raw camel milk of Babile districrt were high. The most effective antimicrobial agent were cefoxitin, oxacillin and erythromycin. Therefore washing hands and milking containers before milking and use of stainless steel containers instead of plastic containers could be applied. Raw camel milk intended for human consumption should be,subjected to heat treatment.